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superscript® iii reverse transcriptase synthesis kit  (Thermo Fisher)


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    Thermo Fisher superscript® iii reverse transcriptase synthesis kit
    Superscript® Iii Reverse Transcriptase Synthesis Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/superscript+reverse+transcriptase+iii+kit/superscript++iii+reverse+transcriptase+synthesis+kit/pmc12221670-72-18-24
    Average 90 stars, based on 1 article reviews
    superscript® iii reverse transcriptase synthesis kit - by Bioz Stars, 2026-09
    90/100 stars

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    Related Articles

    Reverse Transcription:

    Article Title: Epigenetic Alteration of Donor Cells with Histone Deacetylase Inhibitor m-Carboxycinnamic Acid Bishydroxymide Improves the In Vitro Developmental Competence of Buffalo (Bubalus bubalis) Cloned Embryos.
    Article Snippet: Epigenetic reprogramming is an indispensable process during the course of mammalian development, but aberrant in cloned embryos.. The aim of this study was to examine the effect of donor cell treatment with histone deacetylase (HDAC) inhibitor m-carboxycinnamic acid bishydroxymide (CBHA) on cloned embryo development and establish its optimal concentration.. Different concentrations of CBHA (2.5, 5.0, 10.0, and 20.0 lM) were used to treat buffalo adult fibroblast cells for 24 hours and effect on cell proliferation, gene expression, and histone modifications was analyzed.

    Article Title: Altered microbiota by a high-fat diet accelerates lethal myeloid hematopoiesis associated with systemic SOCS3 deficiency
    Article Snippet: .. Reverse transcription of RNA to cDNA was performed using the SuperScript Reverse Transcriptase III kit (Thermo Fisher Scientific, MA, USA) with random hexamer primers. .. Each cDNA sample was analyzed using quantitative PCR with the StepOnePlusTM Real-Time PCR System (Thermo Fisher Scientific).

    Article Title: Successful cloning of a superior buffalo bull
    Article Snippet: .. RNA was isolated from 1 × 10 5 cells or blastocysts (n = 3 to 4) from three different replicates using the RNAqueous micro kit (Cat no # AM1931, Ambion, Austin, TX, USA) and subsequently converted to cDNA using superscript reverse transcriptase III kit (Cat no # 18080-051, Invitrogen). .. Real-time PCR based gene expression analysis was performed using the optimized primers (Supplementary Table ) on a step one plus real-time system (Applied Biosystems, Foster City, CA, USA) with PowerUpTM SYBR® green master mix (Cat no # A25742, Invitrogen) at the following thermal cycling conditions: 50 °C for 2 min, 95 °C for 2 min, followed by 40 PCR cycles of 95 °C for 15 sec, 58 °C for 15 sec, and 72 °C for 15 sec. For ensuring specific gene amplification, a melting curve was determined for each gene and 2% agarose electrophoresis was carried out to determine the length of the amplified PCR products. β-actin mRNA was employed as an internal control for the analysis of relative transcript levels of each gene and in negative controls (H 2 O replaced with cDNA in the reaction tubes).

    Article Title: Biomimetic smoking robot for in vitro inhalation exposure compatible with microfluidic organ chips.
    Article Snippet: Exposure of lung tissues to cigarette smoke is a major cause of human disease and death worldwide.. Unfortunately, adequate model systems that can reliably recapitulate disease biogenesis in vitro, including exposure of the human lung airway to fresh whole cigarette smoke (WCS) under physiological breathing airflow, are lacking.. This protocol extension builds upon, and can be used with, our earlier protocol for microfabrication of human organs-on-chips.

    Article Title: Enhanced anaerobic digestion of dairy wastewater in a granular activated carbon amended sequential batch reactor
    Article Snippet: RNA purification was carried out using the TURBO DNA‐freeTM Kit (Ambion, Dublin, Ireland) in accordance with the manufacturer's instructions. .. Complementary deoxyribonucleic acid (cDNA) was generated from DNA‐free RNA samples using the superscript reverse transcriptase III kit (Invitrogen, Dublin, Ireland) with random hexamer primers following the manufacturer's instructions. .. Both DNA and cDNA were purified using sodium acetate precipitation (Thermo Fisher Scientific, Dublin, Ireland).

    Article Title: Reduced chondroitin sulfate content prevents diabetic neuropathy through transforming growth factor-β signaling suppression
    Article Snippet: Total RNA was isolated from DRGs using an RNeasy Mini kit (Qiagen, Hilden, Germany), according to the manufacturer’s instructions. .. Reverse transcription of RNA to cDNA was performed using a SuperScript Reverse Transcriptase III kit (Thermo Fisher Scientific) with random hexamer primers. .. Each cDNA sample was analyzed using quantitative PCR with the StepOnePlus Real-Time PCR System (Thermo Fisher Scientific).

    Article Title: Development of an enhanced chain elongation process for caproic acid production from waste-derived lactic acid and butyric acid
    Article Snippet: RNA purification was carried out using the TURBO DNA-freeTM Kit (Ambion by Life Technology) in accordance with the manufacturer’s instructions. .. Complementary deoxyribonucleic acid (cDNA) was generated from DNA-free RNA samples using the superscript reverse transcriptase III kit (Invitrogen) following the manufacturer’s recommendations. .. Both DNA and cDNA were purified using a sodium acetate precipitation (Thermo fisher scientific).

    Random Hexamer:

    Article Title: Altered microbiota by a high-fat diet accelerates lethal myeloid hematopoiesis associated with systemic SOCS3 deficiency
    Article Snippet: .. Reverse transcription of RNA to cDNA was performed using the SuperScript Reverse Transcriptase III kit (Thermo Fisher Scientific, MA, USA) with random hexamer primers. .. Each cDNA sample was analyzed using quantitative PCR with the StepOnePlusTM Real-Time PCR System (Thermo Fisher Scientific).

    Article Title: Enhanced anaerobic digestion of dairy wastewater in a granular activated carbon amended sequential batch reactor
    Article Snippet: RNA purification was carried out using the TURBO DNA‐freeTM Kit (Ambion, Dublin, Ireland) in accordance with the manufacturer's instructions. .. Complementary deoxyribonucleic acid (cDNA) was generated from DNA‐free RNA samples using the superscript reverse transcriptase III kit (Invitrogen, Dublin, Ireland) with random hexamer primers following the manufacturer's instructions. .. Both DNA and cDNA were purified using sodium acetate precipitation (Thermo Fisher Scientific, Dublin, Ireland).

    Article Title: Reduced chondroitin sulfate content prevents diabetic neuropathy through transforming growth factor-β signaling suppression
    Article Snippet: Total RNA was isolated from DRGs using an RNeasy Mini kit (Qiagen, Hilden, Germany), according to the manufacturer’s instructions. .. Reverse transcription of RNA to cDNA was performed using a SuperScript Reverse Transcriptase III kit (Thermo Fisher Scientific) with random hexamer primers. .. Each cDNA sample was analyzed using quantitative PCR with the StepOnePlus Real-Time PCR System (Thermo Fisher Scientific).

    Isolation:

    Article Title: Successful cloning of a superior buffalo bull
    Article Snippet: .. RNA was isolated from 1 × 10 5 cells or blastocysts (n = 3 to 4) from three different replicates using the RNAqueous micro kit (Cat no # AM1931, Ambion, Austin, TX, USA) and subsequently converted to cDNA using superscript reverse transcriptase III kit (Cat no # 18080-051, Invitrogen). .. Real-time PCR based gene expression analysis was performed using the optimized primers (Supplementary Table ) on a step one plus real-time system (Applied Biosystems, Foster City, CA, USA) with PowerUpTM SYBR® green master mix (Cat no # A25742, Invitrogen) at the following thermal cycling conditions: 50 °C for 2 min, 95 °C for 2 min, followed by 40 PCR cycles of 95 °C for 15 sec, 58 °C for 15 sec, and 72 °C for 15 sec. For ensuring specific gene amplification, a melting curve was determined for each gene and 2% agarose electrophoresis was carried out to determine the length of the amplified PCR products. β-actin mRNA was employed as an internal control for the analysis of relative transcript levels of each gene and in negative controls (H 2 O replaced with cDNA in the reaction tubes).

    Pore Size:

    Article Title: Biomimetic smoking robot for in vitro inhalation exposure compatible with microfluidic organ chips.
    Article Snippet: Exposure of lung tissues to cigarette smoke is a major cause of human disease and death worldwide.. Unfortunately, adequate model systems that can reliably recapitulate disease biogenesis in vitro, including exposure of the human lung airway to fresh whole cigarette smoke (WCS) under physiological breathing airflow, are lacking.. This protocol extension builds upon, and can be used with, our earlier protocol for microfabrication of human organs-on-chips.

    Membrane:

    Article Title: Biomimetic smoking robot for in vitro inhalation exposure compatible with microfluidic organ chips.
    Article Snippet: Exposure of lung tissues to cigarette smoke is a major cause of human disease and death worldwide.. Unfortunately, adequate model systems that can reliably recapitulate disease biogenesis in vitro, including exposure of the human lung airway to fresh whole cigarette smoke (WCS) under physiological breathing airflow, are lacking.. This protocol extension builds upon, and can be used with, our earlier protocol for microfabrication of human organs-on-chips.

    SYBR Green Assay:

    Article Title: Biomimetic smoking robot for in vitro inhalation exposure compatible with microfluidic organ chips.
    Article Snippet: Exposure of lung tissues to cigarette smoke is a major cause of human disease and death worldwide.. Unfortunately, adequate model systems that can reliably recapitulate disease biogenesis in vitro, including exposure of the human lung airway to fresh whole cigarette smoke (WCS) under physiological breathing airflow, are lacking.. This protocol extension builds upon, and can be used with, our earlier protocol for microfabrication of human organs-on-chips.

    Microarray:

    Article Title: Biomimetic smoking robot for in vitro inhalation exposure compatible with microfluidic organ chips.
    Article Snippet: Exposure of lung tissues to cigarette smoke is a major cause of human disease and death worldwide.. Unfortunately, adequate model systems that can reliably recapitulate disease biogenesis in vitro, including exposure of the human lung airway to fresh whole cigarette smoke (WCS) under physiological breathing airflow, are lacking.. This protocol extension builds upon, and can be used with, our earlier protocol for microfabrication of human organs-on-chips.

    Radio Immunoprecipitation:

    Article Title: Biomimetic smoking robot for in vitro inhalation exposure compatible with microfluidic organ chips.
    Article Snippet: Exposure of lung tissues to cigarette smoke is a major cause of human disease and death worldwide.. Unfortunately, adequate model systems that can reliably recapitulate disease biogenesis in vitro, including exposure of the human lung airway to fresh whole cigarette smoke (WCS) under physiological breathing airflow, are lacking.. This protocol extension builds upon, and can be used with, our earlier protocol for microfabrication of human organs-on-chips.

    Lysis:

    Article Title: Biomimetic smoking robot for in vitro inhalation exposure compatible with microfluidic organ chips.
    Article Snippet: Exposure of lung tissues to cigarette smoke is a major cause of human disease and death worldwide.. Unfortunately, adequate model systems that can reliably recapitulate disease biogenesis in vitro, including exposure of the human lung airway to fresh whole cigarette smoke (WCS) under physiological breathing airflow, are lacking.. This protocol extension builds upon, and can be used with, our earlier protocol for microfabrication of human organs-on-chips.

    Extraction:

    Article Title: Biomimetic smoking robot for in vitro inhalation exposure compatible with microfluidic organ chips.
    Article Snippet: Exposure of lung tissues to cigarette smoke is a major cause of human disease and death worldwide.. Unfortunately, adequate model systems that can reliably recapitulate disease biogenesis in vitro, including exposure of the human lung airway to fresh whole cigarette smoke (WCS) under physiological breathing airflow, are lacking.. This protocol extension builds upon, and can be used with, our earlier protocol for microfabrication of human organs-on-chips.

    Generated:

    Article Title: Enhanced anaerobic digestion of dairy wastewater in a granular activated carbon amended sequential batch reactor
    Article Snippet: RNA purification was carried out using the TURBO DNA‐freeTM Kit (Ambion, Dublin, Ireland) in accordance with the manufacturer's instructions. .. Complementary deoxyribonucleic acid (cDNA) was generated from DNA‐free RNA samples using the superscript reverse transcriptase III kit (Invitrogen, Dublin, Ireland) with random hexamer primers following the manufacturer's instructions. .. Both DNA and cDNA were purified using sodium acetate precipitation (Thermo Fisher Scientific, Dublin, Ireland).

    Article Title: Development of an enhanced chain elongation process for caproic acid production from waste-derived lactic acid and butyric acid
    Article Snippet: RNA purification was carried out using the TURBO DNA-freeTM Kit (Ambion by Life Technology) in accordance with the manufacturer’s instructions. .. Complementary deoxyribonucleic acid (cDNA) was generated from DNA-free RNA samples using the superscript reverse transcriptase III kit (Invitrogen) following the manufacturer’s recommendations. .. Both DNA and cDNA were purified using a sodium acetate precipitation (Thermo fisher scientific).



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